Preparation and handling of aequorin solutions for the measurement of cellular Ca2+ |
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Authors: | O Shimomura |
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Institution: | Marine Biological Laboratory, Woods Hole, Massachusetts. |
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Abstract: | Main characteristics of the various types of aequorin presently available for measuring cellular Ca2+, i.e. heterogeneous aequorin, isoaequorins, recombinant aequorin, fluorescein-labeled aequorin and semi-synthetic aequorins, are summarized. Basic techniques of preparing and handling the solutions of those aequorins for measuring Ca2+, including such techniques as concentrating aequorin solutions, freeze-drying, changing buffer composition, and the regeneration of active aequorin, are described. |
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