Replication of the R6K plasmid during the Escherichia coli cell cycle. |
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| Authors: | J D Keasling B O Palsson S Cooper |
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| Affiliation: | Department of Chemical Engineering, University of Michigan, Ann Arbor 48109-2136. |
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| Abstract: | The cell-cycle replication pattern of the R6K plasmid has been investigated by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The high-copy plasmid R6K replicates exponentially in a cell-cycle-independent manner. A mini-R6K plasmid deleted for the ori alpha origin of replication also replicates, exponentially in a cell-cycle-independent manner. |
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