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KaryoCreate: A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres
Affiliation:1. Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York, NY, USA;2. Department of Experimental Oncology, IEO European Institute of Oncology IRCCS, Milan, Italy;3. Department of Biology and Biotechnology Charles Darwin, University of Rome “La Sapienza”, 00185 Rome, Italy;4. Department of Pathology and Laura & Isaac Perlmutter Cancer Center, NYU School of Medicine, New York, NY, USA;5. Moores Cancer Center, University of California, San Diego, 3855 Health Sciences Drive, La Jolla, CA 92093, USA;6. Department of Cell Biology, NYU Langone Health, New York, NY, USA;7. Department of Oncology and Hemato-Oncology, University of Milan, 20141 Milan, Italy;1. Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland;2. Department of Ophthalmology, University of Basel, Basel, Switzerland;3. Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland;4. Two-Photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary;1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA;2. MRC Cancer Unit, University of Cambridge, Hutchison Research Centre, Cambridge Biomedical Campus, Cambridge CB2 0XZ, UK;3. Department of Nutritional Sciences, Rutgers School of Environmental and Biological Sciences, The State University of New Jersey, New Brunswick, NJ 08901, USA;4. Salk Institute for Biological Studies, La Jolla, CA 92037, USA;5. University of Cambridge, Cancer Research UK Cambridge Institute, Li Ka Shing Centre, Cambridge CB2 0RE, UK;6. Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA;7. Kaiser Permanente Northern California Division of Research, Oakland, CA 94612, USA;8. Wellcome Trust-MRC Institute of Metabolic Science and MRC Metabolic Diseases Unit, University of Cambridge, Cambridge CB2 0QQ, UK;9. University of Rochester Medical Center, University of Rochester, Rochester, NY 14642, USA;10. Division of Endocrinology, Department of Medicine, Weill Cornell Medicine, New York, NY 10021, USA;11. Ruprecht Karl University of Heidelberg, Heidelberg 69117, Germany;12. Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA;13. Department of Medical Oncology, University College London Hospitals, London WC1E 6DD, UK;14. Cancer Research UK Lung Cancer Centre of Excellence and Cancer Metastasis Laboratory, University College London Cancer Institute, London WC1E 6DD, UK;15. The Beatson Institute for Cancer Research, Cancer Research UK, Glasgow G61 1BD, UK;16. Department of Genetics, Blavatnik Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA;17. Rutgers Cancer Institute of New Jersey, Department of Molecular Biology and Biochemistry, Rutgers University, The State University of New Jersey, New Brunswick, NJ 08901, USA;18. Ludwig Princeton Branch, Ludwig Institute for Cancer Research, Princeton University, Princeton, NJ 08544, USA;19. Northwell Health Cancer Institute, Northwell Health, New Hyde Park, NY 11042, USA;1. State Key Laboratory of Membrane Biology, Peking-Tsinghua Joint Center for Life Sciences, School of Life Sciences, Peking University, Beijing 100871, China;2. Changping Laboratory, Beijing 102206, China;3. Changping Laboratory Graduate Program, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China;4. MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China;5. National Institute of Biological Sciences, Beijing 102206, China;6. Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 100084, China;7. National Biomedical Imaging Center, Peking University, Beijing 100871, China;1. State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China;2. Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;3. Department of Mathematics, Shanghai Normal University, Shanghai 200234, China;4. Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China;5. Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children’s Hospital, and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA;6. School of Life Sciences, Fudan University, Shanghai 200438, China;7. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;8. Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, 141-83 Stockholm, Sweden;9. Kindstar Global Precision Medicine Institute, Wuhan 430000, China;10. School of Biosciences, University of Chinese Academy of Sciences, Beijing 101499, China;11. Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China;12. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden;13. State Key Laboratory of Farm Animal Biotech Breeding, China Agricultural University, Beijing 100193, China;14. Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China;15. Center for Immune-Related Diseases at Shanghai Institute of Immunology, Department of Endocrinology and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;16. Shanghai Huashen Institute of Microbes and Infections, Shanghai 200052, China;1. Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA;2. Department of Pathology, Center for Cell Reprogramming, Georgetown University Medical Center, Washington, DC 20007, USA;3. Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA;1. Biomedical Pioneering Innovative Center and School of Life Sciences, Peking University, Beijing 100871, China;2. Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China;3. Changping Laboratory, Beijing, China
Abstract:
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  • Keywords:aneuploidy  centromere  chromosome gains and losses  kinetochore  DNA damage  mitosis  chromosome missegregation  CRISPR  cancer
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