缺苞箭竹种子人工老化及不同大小种子的生理研究

为了解缺苞箭竹(Fargesia denudate Yi)种子的老化机理和不同大小种子间的差异,对其进行了人工老化处理和大、中、小三组种子的区分,并分别对种子活力,总抗氧化能力,酶活性,丙二醛含量和电导率进行测定。发现经人工老化后种子活力显著降低,电导率和丙二醛含量随老化程度加深而上升,总抗氧化能力和过氧化氢酶活力随老化程度加深而下降,超氧化物歧化酶的活力则出现上升的趋势,但超氧化物歧化酶与丙二醛的比值呈现先升后降的趋势。总抗氧化能力、超氧化物歧化酶与丙二醛的比值能够很好的反映种子老化的程度和抗氧化能力的变化;总抗氧化能力、电导率和种子活力有很好的相关性。中等大小种子活力最高,总抗氧化能力和几种酶活性均随种子增大而提高,丙二醛含量和电导率则随种子增大而减小,种子大小是与各指标相关的因素。

The Effects of Accelerated Aging on Fargesia denudata (Gramineae) Seeds and the Physiological Differences among Seeds of Different Sizes

Seeds of Fargesia denudate were treated to accelerated aging and the following indices were measured: seed vigor, T-AOC, activities of anti-oxidative enzymes, contents of MDA, and conductivity, and these indices were also measured on the seeds that differ in size. The results indicated that after different degrees of accelerated aging, the contents of MDA and conductivity gradually increased, while the value of T-AOC and activities of CAT were reduced significantly. The activities of SOD went up, but the ratio of SOD/MDA went down after an initial obvious rise. T-AOC and SOD/MDA could reflect the level of seed aging well, and T-AOC and conductivity had significant correlations with seed vigor. The vigor of middle-sized seeds was higher than that of bigger and smaller seeds. The value of conductivity and contents of MDA went up as the bigger seeds were under determination, and the activities of anti-oxidative enzymes and T-AOC went down during the same process. This indicates that the size of Fargesia denudata seeds correlates with the indices tested in this study.