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Presence of immunoreactive adrenomedullin in human and bovine milk |
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| Authors: | Pio R Martínez A Elsasser T H Cuttitta F |
| Affiliation: | a Department of Cell and Cancer Biology, National Cancer Institute, NIH, Building 10 Room 12N226, 9000 Rockville Pike, Bethesda, MD 20892, USA b Growth Biology Laboratory, USDA, Agricultural Research Service, Beltsville, MD 20705, USA |
| Abstract: | We examined by radioimmunoassay the presence of immunoreactive adrenomedullin (ir-AM) in human and bovine milk. Milk samples displaced 125I-AM from the AM-antiserum in parallel to the standard curve. RP-HPLC revealed a main immunoreactive peak eluting as synthetic AM. Concentrations in human milk ranged between 140 and 404 pg/mL. In cow, the levels of AM were 73.5 ± 3.8 pg/mL. Bovine milk products had AM levels similar to those found in fresh bovine milk. Human milk had growth promoting activity on the human intestinal cell line Int-407 that could be partially blocked with an anti-AM antibody. |
| Keywords: | Adrenomedullin Radioimmunoassay Human milk Bovine milk Intestine 407 |
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