Chromosomal localization of silkworm (Bombyx mori) sericin gene 1 and chymotrypsin inhibitor 13 using fluorescence in situ hybridization

The chromosomal locations of two single-copy genes, Ser-1 and Cl-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5±1.4 position in pachytene; and that Cl-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2±1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper. Supported by the National Natural Science Foundation of China (Grant Nos. 39730730 and 39770574), Foundation for University Leading Teacher by the Ministry of Education of China (Grant No.GG-230-10625-1065), the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, The Ministry of Education of China, and the Science Research Foundation of Ministry of Education of Japan (Grant No. 09045069)