Scanning electron microscopy preparation methods: their influence on the morphology and fibril formation in Pseudomonas fragi (ATCC 4973) |
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| Authors: | T W Fraser A Gilmour † |
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| Institution: | *Department of Agriculture for Northern Ireland;†The Queen's University of Belfast, Agriculture and Food Science Centre, Newforge Lane, Belfast BT9 5 P X, Northern Ireland |
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| Abstract: | The use of cryo-scanning electron microscopy (cryo-SEM) in the study of the morphology of Pseudomonas fragi (ATCC 4973) revealed gross differences when compared to material prepared using conventional chemical methods. Changes associated with each step of the chemical preparatory procedure were monitored by cryo-SEM. It appeared that fibril formation was associated with the ethanol dehydration stage of the chemical preparation method and was not an attachment feature of these cells. |
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