miRNA-20a促进卵巢癌细胞OVCAR3的转移 |
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引用本文: | 韩喆,刘艳坤,姜洁纯,刘民,李欣,汤华.miRNA-20a促进卵巢癌细胞OVCAR3的转移[J].国外医学:分子生物学分册,2009(4):297-301. |
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作者姓名: | 韩喆 刘艳坤 姜洁纯 刘民 李欣 汤华 |
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作者单位: | 天津医科大学基础医学院,天津市生命科学中心实验室,天津市300070 |
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基金项目: | 国家自然科学基金(No.30873017),天津市自然科学基金重点基金项目(No.08JCZDJC23300) |
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摘 要: | 目的检测miRNA.20a对卵巢癌细胞系OVCAR3转移能力的影响。方法通过实时定量RT-PCR验证反义寡核苷酸与小干扰RNA封闭与过表达的效果,然后利用MTF、软琼脂集落形成和transwell侵袭实验检测封闭和过表达miRNA.20a对OVCAR3细胞增殖及转移能力的影响。结果封闭内源性miRNA-20a后,细胞活性基本不受影响,但集落形成能力和细胞的转移能力明显降低。过表达miRNA-20a后,细胞活性基本不受影响,但集落形成能力和细胞的转移能力明显升高。结论miRNA-20a可能参与了卵巢癌细胞OVCAR3的转移。
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关 键 词: | miR-20a 转移 OVCAR3细胞 侵袭实验 |
microRNA-20a Promotes Metastasis of OVCAR3 Ovarian Cancer Cells |
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Authors: | HAN Ze LIU Yankun JIANG Jiechun LIU Min LI Xin TANG Hua |
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Institution: | ( Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, 300070, China) |
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Abstract: | Objective To detect the effect of miR-20a on metastasis. Methods The effects of antisense oligonucleotide and small interfering RNA of miR-20a were confirmed by Real-time PCR. miR-20a was either blocked or overexpressed in human ovarian cancer cell line OVCAR3. Cell pro- liferation was detected with MTF assay and sofl-agarose formation assay. And the impact of miR-20a on cell invasion was detected with transwell invasion assay. Results When miR-20a was sup- pressed, both colony formation activity and invasion activity of OVCAR3 were inhibited. On the con- trary, when miR-20a was overexpressed, OVCAR cells were promoted to form colony and become invasive. Cell growth of OVCAR3 was not subject to either suppression or overexpression of miR- 20a. Conclusion miR-20a contributes to metastasis of OVCAR3 cells. |
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Keywords: | miR-20a metastasis OVCAR3 transwell |
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