Transmembrane arrangement of mitochondrial porin or voltage-dependent anion channel (VDAC) |
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Authors: | Vito De Pinto Ferdinando Palmieri |
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Institution: | (1) Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari and CNR Unit for the Study of Mitochondria, Trav. 200 v. Re David, 4, 1-70125 Bari, Italy |
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Abstract: | Porin or voltage-dependent anion-selective channel (VDAC) is the main protein responsible for the high permeability of the outer mitochondrial membrane. The mitochondrial porin is mainly composed of sided -strands, in analogy with bacterial porin, whose structure has been resolved at 1.8 Å resolution. In mitochondrial porins the N-terminal region forms an amphipathic -helix, a structure conserved in organisms very distant from the evolutionary point of view. This part of the protein is exposed to the water phase, as demonstrated by ELISA assays. Various extramembranous loops have been identified by specific proteolytic cleavages. These overall, combined results were used to draw a model of the transmembrane arrangement of mammalian porin. This model is compared to other mitochondrial and bacterial porin models. |
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Keywords: | Porin VDAC (voltage-dependent anion-selective channel) transmembrane arrangement -strands" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-strands mitochondria |
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