烟草病程相关蛋白基因启动子的克隆及序列分析

在植物的防御反应中，会诱导产生一些宿主编码的蛋白，叫做病程相关蛋白。该文通过PCR扩增，从烟草(Nicotiana tobacwn ce.Samsun)中克隆了水扬酸诱导表达的PR—1a基因的两个启动子TP12及TP13。以期用于构建诱导表达基因敲除系统，并用于无性繁殖植物的无标记基因转化。序列分析表明，启动子TP12含1313个核苷酸，与已报道的序列比较，核苷酸的同源性为98．6％；TP13含654个核苷酸，与已报道的序列比较，核苷酸的同源性为99．4％。

Molecular Cloning and Sequences Analysis of Promoters for the Pathogenesis-Related Protein-1a Gene from Tobacco

During the defense reaction of the plant,several host-encoded proteins are induced which are known as pathogenesis-related proteins (PR proteins) and which are believed to play a role in restricting the pathogen.For constructed the induced gene knock out system to use into markers free transgenic plant,two upstream regulatory regions of the pathogenesis-related protein-1a gene were amplified from Nicotiana tabacum cv. Samsun genomic DNA by polymerase chain reaction.After cloning into T vector pUCm-T,these two fragments were sequenced.The results indicated that the cloned fragment TP12 contained 1313 nucleotides,and shared a sequence homology of 98.6% with that from Genbank accession number X76982(PR1a-1533/N-3). The fragment TP13 contained 654 nucleotides,and shared a sequence homology of 99.4% with the sequence of Genbank accession number X76983(PR1a-1533/X).