| 杆状病毒蛋白激酶的特性和核心蛋白的磷酸化及去磷酸化 |
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| 引用本文： | 王Xun章 龙綮新. 杆状病毒蛋白激酶的特性和核心蛋白的磷酸化及去磷酸化[J]. 病毒学报, 1993, 9(1): 85-92 |
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| 作者姓名： | 王Xun章 龙綮新 |
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| 作者单位： | 中山大学昆虫学研究所生物防治国家重点实验室，中山大学昆虫学研究所生物防治国家重点实验室，中山大学昆虫学研究所生物防治国家重点实验室，中山大学昆虫学研究所生物防治国家重点实验室，中山大学昆虫学研究所生物防治国家重点实验室 广州 510275，广州 510275，广州 510275，广州 510275，广州 510275 |
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| 摘 要： |
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| 关 键 词： | 杆状病毒 粉纹夜蛾 蛋白激酶 |
CHARACTERIZATION OF A BACULOVIRUS PROTEIN KINASE AND PHOSPHORYLATION AND DE-PHOSPHORYLATION OF THE VIRUS BASIC CORE PROTEIN |
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| Wang Xunzhang Long Qingxin Xie Weidong Pang Yi Pu Zhelong. CHARACTERIZATION OF A BACULOVIRUS PROTEIN KINASE AND PHOSPHORYLATION AND DE-PHOSPHORYLATION OF THE VIRUS BASIC CORE PROTEIN[J]. Chinese journal of virology, 1993, 9(1): 85-92 |
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| Authors: | Wang Xunzhang Long Qingxin Xie Weidong Pang Yi Pu Zhelong |
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| Abstract: | Cell-released and polyhedron-derived forms of Trichoplusia ni Nuclear Polyhedrosis Virus ( TnNPV)particles possess protein kinase activity and the activity was not enhanced by cyclic nucleotide. The Mg++ cation and pH optima for the protein kinase activity,which catalyzes the pho sphate bound to serine and threonine, was determined and found to be not particularly stringent. Phosphorylation of the virus basie core protein were also found in this study, regardless of whether the kinases used were from virus particles or infected cell extracts and whether phosphorylation was carried out in vitro or in vivo.The demonstration that phosphory1ation of the basie protein occured in most cases but not detected in purified [32p] orthophosphate-labelled virus suggests that de-phosphorylation of the protein takes place before virus packaging. The role for de-phosphorylation of the basie protein is discussed. |
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| Keywords: | Baculovirus Trichoplusia ni Protein kinase Basic core protein Phosphorylation |
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