Enzyme assays using permeabilized cells of Neurospora. |
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| Authors: | J R Basabe C A Lee R L Weiss |
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| Affiliation: | Department of Chemistry, University of California, Los Angeles, California 90024 USA |
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| Abstract: | A procedure is described for the preparation of homogeneous, steady-state cultures of germinated conidia of Neurospora crassa. Permeabilization of such cells has been accomplished by a combination of toluene-ethanol and freeze-thaw treatments. These permeabilized cells have been used for the determination of enzyme activities. The method has been shown to be rapid and rellable and is applicable to nuclear, mitochondrial, and cytosolic enzymes. |
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| Keywords: | To whom requests for reprints should be addressed. |
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