Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label |
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| Authors: | Lucia E Duinhouwer Bernard J M van Rossum Sandra T van Tiel Ramon M van der Werf Gabriela N Doeswijk Joost C Haeck Elwin W J C Rombouts Mari?tte N D ter Borg Gyula Kotek Eric Braakman Jan J Cornelissen Monique R Bernsen |
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| Institution: | 1. Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.; 2. Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.; 3. Department of Nuclear Medicine, Erasmus University Medical Centre, Rotterdam, The Netherlands.; French Blood Institute, FRANCE, |
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| Abstract: | Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34+ cells, with 19F-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34+ cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied. |
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