不同生长期罗汉果蛋白组图谱比较研究

目的:分析、比较30d和60d两个不同时期罗汉果蛋白质组图谱,寻找与罗汉果发育、成熟及罗汉果甜甙生成密切相关基因,为罗汉果的开发和品种改良提供基础。方法:采用双向电泳(2-DE)技术构建30d和60d罗汉果蛋白质组图谱,应用Im-ageMaster 2D图像分析软件对所得蛋白质组图谱进行分析。结果:30d和60d两个不同时期罗汉果蛋白质组图谱有明显差别,与30d罗汉果蛋白组图谱比较,随果实发育、成熟,60d罗汉果有29个新的蛋白产生,30个蛋白消失,16个蛋白表达3倍升高和15个蛋白表达50%下调。结论:罗汉果生长、发育和成熟的不同阶段受特定基因的表达调控。

Comparison of Siraitia grosvenorii Fruit Proteome of Different Growth Periods

Objective:Exploring genes which were related with Siraitia grosvenorii growth,mature and sweet mogroside production by analysis and comparison of proteome maps of 30 and 60 day-old Siraitia grosvenorii fresh fruit.So that basic information was obtained for the exploiture and breed improvement of Siraitia grosvenorii.Methods: The proteome maps of 30d and 60d Siraitia grosvenorii fruit were made by two dimensional gel electrophoresis(2-DE),and then,analyzed by ImageMaster 2D Software.Results: The proteome maps were obviously different in the two different growth period fruit.Compared with proteome map of 30d fruit,29 proteins newly appeared,30 proteins disappeared,16 proteins were 3 times higher and 15 proteins were 50% lower in 60d fruit.Conclusion: Siraitia grosvenorii fruit of different growth and mature periods are regulated by particular genes expression.