Diverse non-mycorrhizal fungal endophytes inhabiting an epiphytic,medicinal orchid (<Emphasis Type="Italic">Dendrobium nobile</Emphasis>): estimation and characterization |
| |
Authors: | Zhi-lin Yuan Yi-cun Chen Yun Yang |
| |
Institution: | (1) Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, China;(2) Institute of Biotechnology, Zhejiang University, Hangzhou, China;(3) Nabanhe National Nature Reserve, Xishuangbanna, Yunan Province, China |
| |
Abstract: | Although the terrestrial and temperate orchids–fungal biology have been largely explored, knowledge of tropical epiphytic
orchids–fungus relationships, especially on the ecological roles imparted by non-mycorrhizal fungal endophytes, is less known.
Exploitation of the endophytic fungal mycobiota residing in epiphytic orchid plants may be of great importance to further
elucidate the fungal ecology in this special habitat as well as developing new approaches for orchid conversations. The composition
of fungal endophytes associated with leaves, stems and roots of an epiphytic orchid (Dendrobium nobile), a famous Chinese traditional medicinal plant, was investigated. Microscopic imaging, culture-dependant method and molecular
phylogeny were used to estimate their entity and diversity. Totally, there were 172 isolates, at least 14 fungal genera and
33 different morphospecies recovered from 288 samples. Ascomycetes, coelomycetes and hyphomycetes were three major fungal
groups. There were higher overall colonization and isolation rates of endophytic fungi from leaves than from other tissues.
Guignardia mangiferae was the dominant fungal species within leaves; while the endophytic Xylariaceae were frequently observed in all plant tissues; Colletotrichum, Phomopsis and Fusarium were also frequently observed. Phylogenetic analysis based on ITS gene revealed the high diversity of Xylariacea fungi and relatively diverse of non-Xylariacea fungi. Some potentially promising beneficial fungi such as Clonostachys rosea and Trichoderma chlorosporum were found in roots. This is the first report concerning above-ground and below-ground endophytic fungi community of an epiphytic
medicinal orchid, suggesting the ubiquitous distribution of non-mycorrhizal fungal endophytes in orchid plants together with
heterogeneity and tissue specificity of the endophyte assemblage. Possible physiological functions played by these fungal
endophytes and their potential applications are also discussed briefly.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Fungal endophytes Dendrobium nobile Xylaria spp Guignardia mangiferae Clonostachys rosea Trichoderma chlorosporum Phylogenetic analysis |
本文献已被 SpringerLink 等数据库收录! |
|