The accumulation of alpha-zein in transgenic tobacco endosperm is stabilized by co-expression of beta-zein |
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Authors: | Coleman Craig E Yoho Paula Randall Escobar Sonja Ogawa Masahiro |
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Institution: | Department of Plant and Animal Sciences, Brigham Young University, Provo, UT 84602, USA. craig_coleman@byu.edu |
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Abstract: | The cysteine-poor alpha-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with delta-zein, whereas the hydrophobic cysteine-rich beta- and gamma-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, alpha-zein was unstable unless co-expressed with gamma-zein. Here we showed that alpha-zein was also stabilized by beta-zein. Small accretions of alpha- and beta-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER. |
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