A modified method of flow cytometric seed screen simplifies the quantification of progeny classes with different ploidy levels |
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Authors: | A Krahulcova J Suda |
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Institution: | (1) Institute of Botany, Academy of Sciences of the Czech Republic, CZ-252 43 Pruhonice, Czech Republic;(2) Department of Botany, Charles University, Benatska 2, CZ-128 01 Prague, Czech Republic |
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Abstract: | Flow cytometric analysis of ten bulked seeds is proposed to quantify particular embryo ploidy classes in Hieracium. The method is recommended 1) for the detection and quantification of residual sexuality in facultative apomicts, which can generate progeny from heteroploid
crosses, 2) for the quantitative screening of pollen donors with different ploidy levels, based on the fertilization success of the
maternal plant, and 3) for the screening of parents producing a high proportion of polyhaploids. |
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Keywords: | facultative apomixis polyhaploids reproduction routes seed progeny screening |
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