| 豆粕水解液为氮源发酵产脂肪酶的研究 |
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| 引用本文： | 朱耀光,谭天伟. 豆粕水解液为氮源发酵产脂肪酶的研究[J]. 生物加工过程, 2008, 6(5): 36-39 |
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| 作者姓名： | 朱耀光 谭天伟 |
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| 作者单位： | 北京化工大学,生命科学与技术学院,北京市生物加工过程重点实验室,北京,100029 |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划)，北京市自然科学基金，北京市科技计划，国家自然科学基金 |
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| 摘 要： | 研究了以豆粕水解液作为氮源，假丝酵母Candida sp．99—125发酵生产脂肪酶的过程。分析水解时间对于产酶的影响，对比豆粕水解前后作为氮源发酵时的产酶规律。在30L发酵罐中批次发酵酶活最高可达6000IU／mL，采用豆油反馈流加之后，发酵脂肪酶活力可达8500IU／mL。
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| 关 键 词： | 脂肪酶 水解 豆粕 |
Lipase production by using hydrolyzed soybean meal as nitrogen source |
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| ZHU Yao-guang,TAN Tian-wei. Lipase production by using hydrolyzed soybean meal as nitrogen source[J]. Chinese Journal of Bioprocess Engineering, 2008, 6(5): 36-39 |
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| Authors: | ZHU Yao-guang TAN Tian-wei |
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| Affiliation: | ( College of Life Science and Technology, Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China) |
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| Abstract: | By using hydrolyzed soybean meal as nitrogen source in Candida sp. 99-125 fermentation its production process was investigated. The effect of hydrolyzing time and differences of lipase production between using soybean meal and hydrolyzed soybean meal as nitrogen source was analyzed. Batch fermentation in a 30 L fermentor was up to high lipase activity of 6 000 IU/mL. By using pH-responsive adding strategy, the final lipase activity reached 8 500 IU/mL. |
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| Keywords: | lipase hydrolyzed soybean meal |
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