| 大肠杆菌AFP111菌体回收连续转化生产丁二酸 |
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| 引用本文： | 吴明科,刘嵘明,梁丽亚,马江锋,陈可泉,姜岷. 大肠杆菌AFP111菌体回收连续转化生产丁二酸[J]. 生物工程学报, 2013, 29(12): 1875-1879 |
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| 作者姓名： | 吴明科 刘嵘明 梁丽亚 马江锋 陈可泉 姜岷 |
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| 作者单位： | 南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室，江苏 南京 211816;南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室，江苏 南京 211816;南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室，江苏 南京 211816;南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室，江苏 南京 211816；中国石化扬子石油化工有限公司研究院，江苏 南京 210048;南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室，江苏 南京 211816;南京工业大学 生物与制药工程学院 材料化学工程国家重点实验室，江苏 南京 211816 |
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| 基金项目： | 国家自然科学基金(No. 21076105)，国家重点基础研究发展计划 (973计划) (No. 2009CB724701)，江苏高校优势学科建设工程项目，国家高科技研究发展计划 (863计划) (2011AA02A203)，新世纪优秀人才支持计划 (No. NCET-12-0732)，江苏省普通高校研究生科研创新计划 (No. CXZZ12_0438) 资助。 |
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| 摘 要： | 在利用大肠杆菌AFP111厌氧发酵生产丁二酸过程中，随着产物丁二酸的不断积累，菌体活力和产酸能力逐渐降低，而通过回收菌体在新鲜培养基中重复发酵，可延长厌氧发酵时间，但是丁二酸生产效率较低。为了提高菌体回收丁二酸的转化效率，通过在回收菌体时有氧诱导 3 h，以纯水为培养基，进行丁二酸转化发酵。在连续进行 3 批次的发酵后，丁二酸的总产量和最终收率分别为 56.50 g/L和90%，生产速率达到了 0.81 g/(L·h)，比未诱导情况下的生产速率提高了13%。
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| 关 键 词： | 大肠杆菌，有氧诱导，丁二酸，菌体回收，发酵 |
| 收稿时间： | 2013-04-20 |
Succinic acid production with Escherichia coli AFP111 recovered from fermentation |
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| Mingke Wu,Rongming Liu,Liya Liang,Jiangfeng M,Kequan Chen and Min Jiang. Succinic acid production with Escherichia coli AFP111 recovered from fermentation[J]. Chinese journal of biotechnology, 2013, 29(12): 1875-1879 |
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| Authors: | Mingke Wu Rongming Liu Liya Liang Jiangfeng M Kequan Chen Min Jiang |
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| Affiliation: | State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology, Nanjing 211816, Jiangsu, China;State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology, Nanjing 211816, Jiangsu, China;State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology, Nanjing 211816, Jiangsu, China;State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology, Nanjing 211816, Jiangsu, China; Nanjing Research Institute of Sinopec Yangzi Petrochemical Company Limited, Nanjing 210048, Jiangsu, China;State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology, Nanjing 211816, Jiangsu, China;State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology, Nanjing 211816, Jiangsu, China |
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| Abstract: | During the anaerobic fermentation by Escherichia coli AFP111 for succinic acid production, the viable cell concentration and productivity were decreased with the raising of succinic acid concentration. In order to restore cellular succinic acid productivity and prolong fermentation time, we collected strains and refreshed medium for repetitive succinic acid production. However, productivity is lower than that in the anaerobic fermentation before reusing strains. To enhance the productivity, strains were aerobically cultivated for 3 h in pure water before anaerobic fermentation. The activities of key enzymes were enhanced for better performance in producing succinic acid at anaerobic stage. After three rounds of repetitive fermentations, succinic acid concentration and yield reached to 56.50 g/L and 90% respectively. The succinic acid productivity was 0.81 g/(L·h), which was 13% higher than the repetitive fermentations without aerobic activation of the strains. |
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| Keywords: | Escherichia coli aerobic induction succinic acid cell recover fermentation |
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