Repeated horizontal transfer of P transposons between Scaptomyza pallida and Drosophila bifasciata |
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Authors: | S. Hagemann E. Haring W. Pinsker |
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Affiliation: | (1) Institut für Allgemeine Biologie, AG Genetik, Medizinische Fakultät, Universität Wien, Währingerstr. 17, A-1090 Wien, Austria |
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Abstract: | Two distinct P element subfamilies, designated M-type and O-type, reside in the genome of D. bifasciata. PCR-screening of 65 Drosophila species revealed that only D. bifasciata and its closest relative D. imaii possess O-type elements. Outside the genus, O-type elements were detected in Scaptomyza pallida. Restriction analyses show that the general structure of the O-type elements from S. pallida and D. bifasciata is the same. Sequence divergence turned out to be extremely low (0.43%). These results suggest that the O-type subfamily of D. bifasciata has been received by horizontal transfer from an external source, most probably from the genus Scaptomyza, as has been previously suspected for the M-type family. Since the sequence divergence between M-type elements from S. pallida and D. bifasciata is eighteenfold higher than that between O-type elements, two independent intergeneric transfer events have to be postulated. In order to re-examine the taxonomic status of S. pallida, a partial sequence (489 bp) of the Adh gene was analysed. The data clearly prove that S. pallida has to be placed far outside the D. obscura group. |
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Keywords: | alcohol dehydrogenase molecular phylogeny PCR-screening |
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