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Meiotic chromosome condensation and pairing in Saccharomyces cerevisiae studied by chromosome painting |
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| Authors: | Harry Scherthan Josef Loidl Tillman Schuster Dieter Schweizer |
| Institution: | (1) Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria;(2) Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria;(3) Present address: Erwin-Schroedinger-Strasse, Division of Human Genetics and Human Biology of the University, W-6750 Kaiserslautern, Federal Republic of Germany |
| Abstract: | Non-isotopic high resolution in sity hybridization was applied to cytological preparations of sporulating yeast cells. Ribosomal DNA (rDNA) and chromosome V-specific recombinant lambda clones were used to tag individual chromosomes and chromosome subregions. This allowed the study of chromosome behaviour during early meiotic prophase. It was found that chromatin becomes condensed and homologous DNA sequences then appear to become aligned prior to synaptonemal complex formation.by E.R. Schmidt |
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