The biological response of hairy roots to O2 levels in bioreactors |
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Authors: | P J Weathers B E Wyslouzil K K Wobbe Y J Kim E Yigit |
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Institution: | (1) Biology and Biotechnology, Worcester Polytechnic Institute, 01609 Worcester, Massachusetts;(2) Chemical Engineering, Worcester Polytechnic, 01609 Worcester, Massachusetts;(3) Chemistry and Biochemistry Departments, Worcester Polytechnic Institute, 01609 Worcester, Massachusetts |
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Abstract: | Summary The efficient exchange of gases between roots and their environment is one of the biggest challenges in bioreactor design
for transformed root cultures. Gas-phase reactors can alleviate this problem as well as provide a new tool for studying the
biological response of roots and other differentiated tissues to changes in the gas phase composition. In our comparison of
liquid- and gas-phase reactors, roots grown in liquid (shake flasks or bubble column reactors) are shown to be under hypoxic
stress. Roots grown in a gas-phase reactor (nutrient mist), while not hypoxic, produced 50% less biomass. These results suggest
that the response of the tissues to gas phase composition are complex and need further study. |
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Keywords: | nutrient mist reactor bubble column reactor alcohol dehydrogenase Artemisia annua |
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