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Detection of NASBA amplified bacterial tmRNA molecules on SLICSel designed microarray probes |
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| Authors: | Ott Scheler Lauris Kaplinski Barry Glynn Priit Palta Sven Parkel Kadri Toome Majella Maher Thomas Barry Maido Remm Ants Kurg |
| Institution: | (1) Dept. of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia;(2) Estonian Biocentre, Tartu, Estonia;(3) Dept. of Bioinformatics, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia;(4) Molecular Diagnostics Research Group, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland |
| Abstract: | BackgroundWe present a comprehensive technological solution for bacterial diagnostics using tmRNA as a marker molecule. A robust probe design algorithm for microbial detection microarray is implemented. The probes were evaluated for specificity and, combined with NASBA (Nucleic Acid Sequence Based Amplification) amplification, for sensitivity. |
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