Isolation and chemical characterization of two distinct "link proteins" from bovine nasal cartilage proteoglycan complex |
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Authors: | F Bonnet J P Périn P Jollès |
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Abstract: | Bovine nasal cartilage proteoglycan aggregates have been dissociated and separated by dissociative density gradient centrifugation into proteoglycan sub-units and "link fraction". The latter contained mainly the two "link proteins" as shown by analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The two "link proteins" were then separated by preparative gel electrophoresis under dissociative conditions. Molecular weight and amino acid composition of both proteins are presented. |
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