IAA-overproducer mutants of Hebeloma cylindrosporum Romagnesi mycorrhizal with Pinus pinaster (Ait.) Sol. and P. sylvestris L. in hydroponic culture |
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Authors: | Jan-Erik Nylund Håkan Wallander Björn Sundberg Gilles Gay |
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Institution: | (1) Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Box 7026, S-75007 Uppsala, Sweden;(2) Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-90183 Umeå, Sweden;(3) Unité d'Ecologie Microbienne, Bât. 405, 43, Université Claude-Bernard Lyon 1, Boulevard du 11 Novembre, F-69622 Villeurbanne Cedex, France |
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Abstract: | Indole-3-acetic acid (IAA) is thought to play a role in the regulation of ectomycorrhiza development, and vigorous mycorrhiza formers such as Pisolithus and Laccaria have previously been shown to accumulate large amounts of IAA in the culture medium in vitro, particularly in the presence of tryptophan. Recently, 5-fluoroindole-resistant and IAA-overproducing mutant strains of Hebeloma cylindrosporum Romagnesi have been developed and described by Durand et al. (1992). We have used some of these and corresponding wild-type strains as mycobionts on seedlings of Pinus pinaster (Ait.) Sol. and P. sylvestris L. in semi-hydroponic culture in an attempt to study IAA effects independent of species-specific differences. However, no significant differences between strains were found in host growth rate, shoot carbohydrate concentration, root morphology, root IAA concentration or mycorrhizal biomass. Since previous work showed a stimulation by these and other mutants and strains on mycorrhiza formation in Petri dish and test tube cultures, we assume that a semi-hydroponic culture system prevents the build up of tryptophan of fungal origin, which is most likely a precondition for enhanced IAA production. |
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Keywords: | IAA overproducers Ectomycorrhiza Hebeloma cylindrosporum |
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