BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast |
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Authors: | Juhásová Barbora Mentel Marek Bhatia-Kiššová Ingrid Zeman Igor Kolarov Jordan Forte Michael Polčic Peter |
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Affiliation: | Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic. |
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Abstract: | Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-X(L) are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins. |
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