| 人GM－CSF在链霉菌中分泌表达的研究 |
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| 引用本文： | 刘岱岚,强伯勤,袁建刚,李元,梁植权.人GM－CSF在链霉菌中分泌表达的研究[J].生物化学与生物物理进展,1996,23(1):56-59. |
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| 作者姓名： | 刘岱岚 强伯勤 袁建刚 李元 梁植权 |
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| 作者单位： | 中国医学科学院基础医学研究所;中国医学科学院基础医学研究所;中国医学科学院基础医学研究所;中国医学科学院医药生物技术研究所;中国医学科学院基础医学研究所 |
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| 摘 要： | 对链霉菌表达体系pIJ459/S. lividans TK54中表达外源基因hGM-CSF的可行性进行了探索，分析了影响外源基因表达的某些因素如：SD与ATG之间的距离，时间对表达的诱导等．为获得外源基因的高效表达探索了最适条件，然后利用MEL信号肽与目的基因片段融合，在链霉菌中获得了分泌型hGM-CSF表达产物，其中有1／3直接分泌到培养基中，其纯化产物经TF－1细胞培养及集落形成实验均证实其生物学活性达到5MU／L．
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| 关 键 词： | 集落刺激因子 基因表达 链霉菌 信号肽 |
| 收稿时间： | 1995/2/13 0:00:00 |
| 修稿时间： | 1995/6/10 0:00:00 |
Study on Secretive Expression of hGM-CSF in Streptomyces |
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| Liu Dailan,Qiang Boqin,Yuan Jiarigang,Li Yuan and Liang Zhiquan.Study on Secretive Expression of hGM-CSF in Streptomyces[J].Progress In Biochemistry and Biophysics,1996,23(1):56-59. |
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| Authors: | Liu Dailan Qiang Boqin Yuan Jiarigang Li Yuan and Liang Zhiquan |
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| Institution: | Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences;Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences;Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences;Institute of MedicalBiotechnology, Chinese Academy Of MedicalSciences;Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences |
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| Abstract: | The high level secretive expression of hGM-CSF in streptomyces was obtained.The factors, which can influence the exogenous expression in prokaryoties, such as the distance between SD and ATG, and the features of expression induction in streptomyces,etc. were studied. Then the secreted hGMCSF protein in streptomyces using the signal peptide MEL were obtained. One third of the expression products were secreted. By the experiment of clony stimulating factor formation and cell culture of TF-1 (hGM-CSF dependent cell line), its biological activity determined is 5 MU/L. |
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| Keywords: | GM-CSF gene expression streptomyces signal peptide |
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