Mapping of oxygen tension and cell distribution in a hollow-fiber bioreactor using magnetic resonance imaging |
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| Authors: | Williams S N Callies R M Brindle K M |
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| Institution: | Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom; telephone: 00 44 1223 333674; fax: 00 44 1223 333345. |
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| Abstract: | We mapped the distribution of dissolved oxygen and mammalian cells in a hollow-fiber bioreactor (HFBR) using (19)F NMR T(1) relaxation time imaging measurements on an infused perfluorocarbon probe molecule and diffusion-weighted (1)H NMR imaging of water. This study shows how cell density influences dissolved oxygen concentration in the reactor and demonstrates that NMR can play an important role in defining the biochemical engineering parameters required for optimization of HFBR design and operation. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 56-61, 1997. |
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