PURIFICATION AND PROPERTIES OF THE PROTHORACICOTROPIC HORMONE OF THE SILKWORM, BOMBYX MORI |
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| Authors: | HIROMICHI NAGASAWA AKIRA ISOGAI AKINORI SUZUKI SABURO TAMURA HIRONORI ISHIZAKI |
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| Institution: | Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Biological Insitute, Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464, Japan |
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| Abstract: | A simple and highly reproducible procedure for partial purification of prothoracicotropic hormone (PTTH) was established starting with 96,000 male adult Bombyx heads. Approximately 28,500-fold purification of PTTH was accomplished with a yield of about 50% and 6 ng of the most purified preparation ("highly purified PTTH") caused adult development in a brainless Samia pupa. The peptidal nature of PTTH was reconfirmed through the effects of various enzymatic and chemical treatments on the biological activity of "highly purified PTTH". Gel-filtration indicated the molecular weight of PTTH to be 4,400. |
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