高通量测序技术在肉类掺假检测中的应用进展

肉类掺假现象普遍存在,导致严重的公共卫生风险和侵犯宗教信仰行为.快速、有效、准确和可靠的检测技术是有效监管肉类掺假的关键手段.近年来,基于高通量测序的DNA宏条形码技术发展迅速,具有高通量、高精度和速度快等特点,并且可以实现复杂样品中多个物种的同时检测,因而在肉类及其制品的掺假检测方面具有明显的优势.文中介绍了近20年...

Application and progress in high-throughput sequencing technology for meat adulteration detection

Adulteration in meat products is a widespread issue that could lead to serious threats to public health and religious violations. Technology that offers rapid, sensitive, accurate and reliable detection of meat species is the key to an effectual monitoring and control against meat adulteration. In recent years, high-throughput sequencing-based DNA metabarcoding technology has developed rapidly. With the characteristics of being high-throughput, highly precise and high-speed, this technology can simultaneously identify multiple species in complex samples, thus offering pronounced advantages in the surveillance of adulteration in meat and meat products. Starting with an introduction of the major developments in the high-throughput sequencing technology in the past two decades, this review provides an overview of the technical characteristics and research methods of DNA metabarcoding, summarizes the application of DNA metabarcoding technology in meat adulteration detection over the last few years, discusses the challenges of using DNA metabarcoding technology in the detection of meat adulteration, and provides future prospects on the development of this technology.