Structure and chromosomal localization of DNA sequences related to ribosomal subrepeats in Vicia faba |
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| Authors: | F Maggini R Cremonini C Zolfino G F Tucci R D'Ovidio V Delre C DePace G T Scarascia Mugnozza P G Cionini |
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| Institution: | (1) Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, I-01100 Viterbo, Italy;(2) Dipartimento di Scienze Botaniche della Università, Via L. Ghini 5, I-56124 Pisa, Italy;(3) Dipartimento di Biologia Vegetale della Università, Borgo XX Giugno 74, I-06100 Perugia, Italy |
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| Abstract: | Subrepeating sequences of 325 bp found in the ribosomal intergenic spacer (IGS) of Vicia faba and responsible for variations in the length of the polycistronic units for rRNA were isolated and used as probes for in situ hybridization. Hybridization occurs at many regions of the metaphase chromosomes besides those bearing rRNA genes, namely chromosome ends and all the heterochromatic regions revealed by enhanced fluorescence after quinacrine staining. The DNA homologous to the 325 bp repeats that does not reside in the IGS was isolated, cloned and sequenced. It is composed of tandemly arranged 336 bp elements, each comprising two highly related 168 bp sequences. This structure is very similar to that of the IGS repeats and ca. 75% nucleotide sequence identity can be observed between these and the 168 bp doublets. The most obvious difference lies in the deletion, in the former, of a 14 bp segment from one of the two related sequences. It is hypothesized that the IGS repeats are derived from the 336 bp elements and have been transposed to ribosomal cistrons from other genome fractions. The possible relations between these sequences and others with similar structural features found in other species are discussed.by D. Schweizer |
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