A practical design of an elution system for preparative electrophoresis in polyacrylamide gel. |
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| Authors: | N Marceau R Blais N Balaux |
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| Affiliation: | Department of Molecular Biology, Institute of Microbiology The Hebrew University-Hadassah Medical School Jerusalem, Israel |
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| Abstract: | The previously applied direct spectrophotometric assay of β-lactamase activity toward cephalosporins was found readily applicable to penicillins too. The differential uv absorption spectra of various penicillins and their corresponding penicilloic acids were determined. The appropriate experimental conditions were examined and the spectrophotometric assay seems adequate for the study of several substrates in a mixture. Also this method was found highly suitable for computerized analysis of the kinetic data for the determination of Michaelis constants of the various penicillins. The use of the integrated form of the rate equation for the evaluation of the best estimates of Michaelis constants was found advantageous. |
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