Molecular characterization of a Ran isoform gene up-regulated in shrimp immunity |
| |
Authors: | Han Fang Wang Xiaoqing Wang Zhiyong |
| |
Institution: | a College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, Chinab Key Laboratory of Healthy Mariculture in the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen, 361021, China |
| |
Abstract: | Diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. Ran gene was an important antiviral gene identified from shrimp and its mRNA level was up-regulated in response to viral infection. In this investigation, a Ran isoform gene (named Ran-iso) cDNA was cloned from shrimp, Marsupenaeus japonicus. The full-length cDNA of Ran-iso was 1286 bp, including a 5′-terminal untranslated region (UTR) of 272 bp, 3′-terminal UTR of 366 bp and an open reading frame (ORF) of 648 bp encoding a polypeptide of 215 amino acids. The deduced protein was highly homologous, it shared 90.64%, 84.19%, 81.48% and 67.58% identities with Ran protein of shrimp, honey bee, human and tobacco respectively. Ran-iso gene was constitutively expressed in 6 tissues examined, including gill, hepatopancreas, hemolymph, heart, intestine and muscle. However, Ran-iso was highest expressed in hepatopancreas (p < 0.01), whereas the expressions of other five tissues were equal and relatively low. Time course analysis showed that the expression level of Ran-iso was obviously up-regulated 2.8 times (at 6 h) as much as that in the control in the hepatopancreas challenged by WSSV. This investigation might provide a clue to elucidate the shrimp innate immunity and would be helpful to shrimp disease control. |
| |
Keywords: | Ran-iso Ran isoform UTR untranslated region Bp base pair ORF open reading frame WSSV white spot syndrome virus proPO prophenoloxidase NE nuclear envelope PjRan a shrimp Ran gene PCR polymerase chain reaction RACE rapid amplification of cDNA ends TdT terminal deoxynucleotidyl transferase FBS fetal bovine serum EGFP enhanced green fluorescent protein CT comparative threshold SPSS Statistics Package for Social Science SD standard deviation DAPI diamidino-2-phenylindole RNAi RNA interference |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|