Self-assemble gene delivery system for molecular targeting using nucleic acid aptamer |
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| Authors: | Kurosaki Tomoaki Higuchi Norihide Kawakami Shigeru Higuchi Yuriko Nakamura Tadahiro Kitahara Takashi Hashida Mitsuru Sasaki Hitoshi |
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| Affiliation: | a Department of Hospital Pharmacy, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki 852-8501, Japanb Global COE program, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japanc Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshidashimoadachi-cho, Sakyo-ku, Kyoto, 606-8501, Japand Institute of Integrated Cell-Material Sciences (iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8302, Japan |
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| Abstract: | We have developed a novel vector constructed with pDNA, polyethylenimine (PEI), and mucin 1 (MUC1) aptamer for tumor-targeted gene delivery. The MUC1 aptamer and non-specific aptamer were employed to coat the pDNA/PEI complexes electrostatically and stable nanoparticles were formed. The addition of a non-specific aptamer to the pDNA/PEI complex decreased gene expression in the human lung cancer cell line, A549 cells expressing MUC1 regularly. At the same time, the pDNA/PEI/MUC1 aptamer complex showed higher gene expression than pDNA/PEI/non-specific aptamer complex. Furthermore, the pDNA/PEI/MUC1 aptamer complex showed markedly high gene expression in tumor-bearing mice; thus, pDNA/PEI/MUC1 aptamer complexes are useful as a tumor-targeted gene delivery system with high transfection efficiency. |
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| Keywords: | pDNA, plasmid DNA PEI, polyethylenimine MUC1, mucin 1 polyA, polyadenylic acid polyIC, polyinosinic-polycytidylic acid FBS, fetal bovine serum cDNA, DNA complementary to RNA BSA, bovine serum albumin RLU, relative light units |
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