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Functional analysis of cus operon promoter of Klebsiella pneumoniae using E. coli lacZ assay
Authors:Zahid Nageena  Zulfiqar Soumble  Shakoori Abdul Rauf
Affiliation:
  • School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan
  • Abstract:
    Keywords:PcusRS, cusRS promoter   A.C50, 50% active concentration   pSL-LacZ::Pcus, cusRS fusion construct with lacZ gene in promoterless pSL vector   MIC, Minimum inhibitory concentration   lacZ gene, gene encoding β-Galactosidase enzyme   bp, base pair   RBS, Ribosomal binding site   kDA, kilodalton   PCR, Polymerase chain reaction
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