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Rapid plant regeneration from various explants of Jatropha integerrima
Authors:M. Sujatha  Mukta Dhingra
Affiliation:(1) Department of Genetics and Plant Breeding, Directorate of Oilseeds Research, Rajendranagar, 500 030 Hyderabad, India
Abstract:A simple, rapid and reproducible protocol for direct shoot regeneration from different explants of Jatropha integerrima was developed. Prolific adventitious shoot bud initiation was obtained using a combination of 2.2 or 4.4 mgrM benzyladenine and 4.9 mgrM indole-3-butyric acid (IBA). Reduction of IBA concentration (2.5 mgrM) promoted further development of shoots. Regenerated shoots rooted readily on Murashige and Skoog (MS) medium lacking growth regulators. Plantlets were acclimatized and successfully transferred to pots.
Keywords:adventitious shoots  micropropagation
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