Conformational flexibility of σ70 in anti‐terminator loading |
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Authors: | Pieter L DeHaseth Jonatha M Gott |
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Institution: | 1. RNA Center and;2. Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106‐4973, USA. |
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Abstract: | In promoter DNA, the preferred distance of the ?10 and ?35 elements for interacting with RNA polymerase‐bound σ70 is 17 bp. However, the Devi et al. paper in this issue of Molecular Microbiology demonstrates that when the C‐terminal domain of σ70, including the 3.2 linker, is not attached to the core enzyme, distances between 0 and 3 bp can be accommodated. This attests to the great flexibility of the 3.2 linker. The particularly stable complex with the 2 bp separation may lend itself to structural studies of an early elongation complex containing σ70. |
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