Natural variation in ozone sensitivity among Arabidopsis thaliana accessions and its relation to stomatal conductance |
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Authors: | MIKAEL BROSCHÉ EBE MERILO FLORIAN MAYER PRIIT PECHTER IRINA PUZÕRJOVA GÜNTER BRADER JAAKKO KANGASJÄRVI HANNES KOLLIST |
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Affiliation: | 1. Division of Plant Biology and;2. Institute of Technology, University of Tartu, Nooruse 1, Tartu 50411, Estonia,;3. Lehrstuhl für Botanik, Department Biologie I, Ludwig‐Maximilians‐Universit?t München, Gro?haderner Stra?e 2, 82152 Planegg‐Martinsried, Germany and;4. Division of Genetics, Department of Biosciences, University of Helsinki, FI‐00014 Helsinki, Finland,;5. Austrian Institute of Technology GmbH, Bioresources, Health & Environment Department, 2444 Seibersdorf, Austria |
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Abstract: | Genetic variation between naturally occurring populations provides a unique source to unravel the complex mechanisms of stress tolerance. Here, we have analysed O3 sensitivity of 93 natural Arabidopsis thaliana accessions together with five O3‐sensitive mutants to acute O3 exposure. The variation in O3 sensitivity among the natural accessions was much higher than among the O3‐sensitive mutants and corresponding wild types. A subset of nine accessions with major variation in their O3 responses was studied in more detail. Among the traits assayed, stomatal conductance (gst) was an important factor determining O3 sensitivity of the selected accessions. The most O3‐sensitive accession, Cvi‐0, had constitutively high gst, leading to high initial O3 uptake rate and dose received during the first 30 min of exposure. Analyzing O3‐induced changes in stress hormone concentrations indicated that jasmonate (JA) concentration was also positively correlated with leaf damage. Quantitative trait loci (QTL) mapping in a Col‐0 × Cvi‐0 recombinant inbred line (RIL) population identified three QTLs for O3 sensitivity, and one for high water loss of Cvi‐0. The major O3 QTL mapped to the same position as the water loss QTL further supporting the role of stomata in regulating O3 entry and damage. |
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Keywords: | abscisic acid antioxidants Arabidopsis thaliana natural accessions jasmonate ozone‐sensitive mutants QTL – quantitative trait loci RIL – recombinant inbred line salicylic acid visible injury |
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