In vitro studies of DNA damage and repair mechanisms induced by BNCT in a poorly differentiated thyroid carcinoma cell line |
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| Authors: | C.?Rodriguez,M.?Carpano,P.?Curotto,S.?Thorp,M.?Casal,G.?Juvenal,M.?Pisarev,M.?A.?Dagrosa author-information" > author-information__contact u-icon-before" > mailto:dagrosa@cnea.gov.ar" title=" dagrosa@cnea.gov.ar" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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| Affiliation: | 1.Radiobiology Department (CAC, CNEA),Buenos Aires,Argentina;2.Instrumentation and Control Department (CAE, CNEA),Buenos Aires,Argentina;3.RA-3-Investigation and Production Reactors (CAE, CNEA),Buenos Aires,Argentina;4.Oncology Institute “ángel H. Roffo”-University of Buenos Aires,Ciudad Autónoma de Buenos Aires,Argentina;5.Scientific and Technical Research National Council (CONICET),Ciudad Autónoma de Buenos Aires,Argentina |
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| Abstract: | Boron neutron capture therapy (BNCT) for aggressive tumors is based on nuclear reaction [10B (n, α) 7Li]. Previously, we demonstrated that BNCT could be applied for the treatment of undifferentiated thyroid carcinoma. The aim of the present study was to describe the DNA damage pattern and the repair pathways that are activated by BNCT in thyroid cells. We analyzed γH2AX foci and the expression of Ku70, Rad51 and Rad54, main effector enzymes of non-homologous end joining (NHEJ) and homologous recombination repair (HRR) pathways, respectively, in thyroid follicular carcinoma cells. The studied groups were: (1) C [no irradiation], (2) gamma [60Co source], (3) N [neutron beam alone], (4) BNCT [neutron beam plus 10 µg 10B/ml of boronphenylalanine (10BPA)]. The total absorbed dose was always 3 Gy. The results showed that the number of nuclear γH2AX foci was higher in the gamma group than in the N and BNCT groups (30 min–24 h) (p?p?
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