Arabidopsis homolog of the yeast TREX‐2 mRNA export complex: components and anchoring nucleoporin |
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| Authors: | Qing Lu Xurong Tang Gang Tian Fang Wang Kede Liu Vi Nguyen Susanne E Kohalmi Wilfred A Keller Edward WT Tsang John J Harada Steven J Rothstein Yuhai Cui |
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| Institution: | 1. Agriculture and Agri‐Food Canada, Southern Crop Protection and Food Research Centre, London, ON N5V 4T3, Canada;2. Department of Molecular and Cellular Biology, University of Guelph, ON N1G 2W1, Canada;3. Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, SK S7N 0W9, Canada;4. Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada;5. National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China;6. Section of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA |
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| Abstract: | Nuclear pore complexes (NPCs) are vital to nuclear–cytoplasmic communication in eukaryotes. The yeast NPC‐associated TREX‐2 complex, also known as the Thp1–Sac3–Cdc31–Sus1 complex, is anchored on the NPC via the nucleoporin Nup1, and is essential for mRNA export. Here we report the identification and characterization of the putative Arabidopsis thaliana TREX‐2 complex and its anchoring nucleoporin. Physical and functional evidence support the identification of the Arabidopsis orthologs of yeast Thp1 and Nup1. Of three Arabidopsis homologs of yeast Sac3, two are putative TREX‐2 components, but, surprisingly, none are required for mRNA export as they are in yeast. Physical association of the two Cdc31 homologs, but not the Sus1 homolog, with the TREX‐2 complex was observed. In addition to identification of these TREX‐2 components, direct interactions of the Arabidopsis homolog of DSS1, which is an established proteasome component in yeast and animals, with both the TREX‐2 complex and the proteasome were observed. This suggests the possibility of a link between the two complexes. Thus this work has identified the putative Arabidopsis TREX‐2 complex and provides a foundation for future studies of nuclear export in Arabidopsis. |
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| Keywords: | TREX‐2 mRNA export complex nucleoporin NUP1 THP1 SAC3 DSS1 |
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