| NaCl盐析提取酶解液中Ⅱ型胶原的工艺 |
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| 引用本文： | 曹慧,许时婴.NaCl盐析提取酶解液中Ⅱ型胶原的工艺[J].生物加工过程,2010,8(4):22-26. |
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| 作者姓名： | 曹慧 许时婴 |
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| 作者单位： | 1. 上海理工大学,医疗器械与食品学院,上海,200093
2. 江南大学,食品学院,无锡,214036 |
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| 基金项目： | 上海市高校选拔优秀青年教师科研专项基金 |
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| 摘 要： | 采用响应面分析法对酶解液中Ⅱ型胶原的盐析条件进行优化,并通过SDS-PAGE电泳及圆二色谱对其纯度和结构进行鉴定。结果表明:酶解液中Ⅱ型胶原的最佳盐析条件为NaCl浓度3.5mol/L、22.3℃、31.08h,在此条件下Ⅱ型胶原回收率为90.88%;采用盐析法从酶解液中制备的Ⅱ型胶原,具有较高纯度并保持着完整的3股螺旋结构。
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| 关 键 词： | Ⅱ型胶原 盐析 酶解液 |
Salt-out for extractingtype II collagen from enzyme hydrolysate |
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| CAO Hui,XU Shi-ying.Salt-out for extractingtype II collagen from enzyme hydrolysate[J].Chinese Journal of Bioprocess Engineering,2010,8(4):22-26. |
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| Authors: | CAO Hui XU Shi-ying |
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| Institution: | 1.Medical Equipment and Food Institute,Shanghai University of Science and Technology,Shanghai 200093,China;2.School of Food Science and Technology,Jiangnan University,Wuxi 214036,China) |
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| Abstract: | Response surface methodology was used to optimize technology of salt-out of type II collagen from enzymatic hydrolysate.SDS-PAGE and circular dichroism were used to determine the purity and the structure of type Ⅱ collagen.The results showed that the optimum parameters for NaCl salting out were as follows：extraction time 31.08 h,NaCl concentration 3.5 mol/L,temperature 22.3 ℃,and the recovery ratio 90.88%.Type II collagen with salting-out methodology is essentially free from contaminating proteins and keeps characteristic of triple helical conformation. |
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| Keywords: | type Ⅱ collagen salt-out enzymatic hydrolysate |
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