Genotype-specific response of cultured broccoli (Brassica oleracea var. italica) anthers to cytokinins |
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Authors: | Paul G Arnison Pauline Donaldson Anne Jackson Charmaine Semple Wilf Keller |
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Institution: | (1) Paladin Hybrids Inc., P.O. Box 219, L6V 2L2 Brampton, Ontario, Canada;(2) The Biochemistry Department, The University of Ottawa, 210A, 40 Marie Curie Drive, KIN 9B4 Ottawa, Ontario, Canada;(3) Genetic Engineering Section, Plant Research Centre, Central Experimental Farm, Agriculture Canada, K1A 0C6 Ottawa, Ontario, Canada |
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Abstract: | Anther culture medium was prepared with different types and concentrations of cytokinins to gain greater insight into the control of embryo formation during Brassica oleracea L. var. italica (broccoli) anther culture. The independent addition of the four cytokinins tested had widely divergent effects dependent upon cytokinin concentration and the genetic background of the test plants. All cytokinins were generally inhibitory at high concentrations, however, individual plants showed significant stimulation of embyro formation at typical physiological levels. The influence of cytokinins was highly cultivar-specific, some lines were stimulated, others inhibited and still other test lines were largely unaffected. Although the addition of cytokinins was needed for embryo formation for some plants, in no instance were cytokinins able to replace the inductive effect of high-temperature treatments. |
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Keywords: | Brassica oleracea broccoli anther culture cytokinins |
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