|
|||||
|
|
Overexpression of an alkaline lipase gene from Proteus vulgaris K80 in Escherichia coli BL21/pKLE |
|
| Authors: | Hong-Weon Lee Sun-Jun Yoon Hyung-Kwoun Kim Kyong-Mun Park Tae-Kwang Oh Joon-Ki Jung |
| Affiliation: | (1) Bio-Venture Center, Korea;(2) Environmental Bioresources Laboratory, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon, 305-600, Korea;(3) Chemicals & Biotechnology Industries Division, Ministry of Commerce Industry & Energy, Kwacheon, 427-760, Korea |
| Abstract: | Over-expression of Proteus vulgaris K80 lipase gene in Escherichia coli BL21 (DE3)/pKLE was achieved, with the enzyme being produced in an as active soluble form, using the T7 RNA polymerase system in a modified M9 salt or M9ZB medium. d-Lactose (55 mM) was used to induce gene expression and gave twice the lipase activity achieved with 0.4 mM IPTG. The expression of the lipase gene depended on the feeding rate of glucose being optimal at 12 g l–1 h–1. |
| Keywords: | E. coli BL21/pKLE lipase overexpression |
| 本文献已被 SpringerLink 等数据库收录！ | |