Coupling of polyphosphoinositide breakdown with calcium efflux in formyl-methionyl-leucyl-phenylalanine-stimulated rabbit neutrophils |
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Authors: | K Yano S Nakashima Y Nozawa |
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Institution: | Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan |
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Abstract: | Exposure of rabbit neutrophils to formyl-methionyl-leucyl-phenylalanine (FMLP) induced the efflux of 45Ca2+ from pre-labeled cells which was almost complete within 30 s. On the other hand, FMLP-induced 45Ca2+ influx did not become apparent until 60 s after stimulation. When 3H]arachidonic acid-labeled neutrophils were stimulated with FMLP, the radioactivities in phosphatidylinositol 4,5-biphosphate (TPI) and phosphatidylinositol 4-phosphate (DPI) significantly decreased in parallel with the induction of 45Ca2+ efflux. In contrast, degradation of polyphosphoinositides in 3H]glycerol-labeled neutrophils was not significant until 60 s. Taken together, these results indicate that the early degradation of polyphosphoinositides, especially of those rich in arachidonic acid is closely associated with the initial efflux of calcium in FMLP-stimulated rabbit neutrophils. The study of resynthesis of polyphosphoinositides by measuring 32Pi incorporation into these lipids is also presented. |
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Keywords: | Polyphosphoinositide Formyl-methionyl-leucyl-phenylalanine Rabbit neutrophil TPI phosphatidylinositol 4 5-biphosphate DPI phosphatidylinositol 4-phosphate PI phosphatidylinositol PA phosphatidic acid FMLP formyl-methionyl-leucyl-phenylalanine PBS phosphate-buffered saline EDTA ethylenediaminetetraacetic acid HPTLC high-performance thin-layer chromatography |
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