| 番红花EST资源的SSR信息分析 |
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| 引用本文： | 陈国庆.番红花EST资源的SSR信息分析[J].广西植物,2011,31(1):43-46. |
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| 作者姓名： | 陈国庆 |
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| 作者单位： | 湖北师范学院,生命科学学院,湖北,黄石,435002 |
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| 基金项目： | 国家自然科学基金，湖北省教育厅青年项目，湖北师范学院人才引进项目 |
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| 摘 要： | 从NCBI公共数据库下载得到6745条番红花EST,通过前处理得到全长为612.01 kb的无冗余EST 1431条.在这些序列中搜索出108个SSR,分布于103条EST中,出现频率为7.55%.这些EST-SSR的平均分布距离是5.67 kb.二核苷酸重复和三核苷酸重复是番红花主要的重复类型,分别占总EST-SSR...
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| 关 键 词： | 番红花 药用植物 EST EST-SSR 微卫星 |
Analysis of SSR information in EST resource
of saffron crocus(Crocus sativus) |
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| CHEN Guo-Qing.Analysis of SSR information in EST resource
of saffron crocus(Crocus sativus)[J].Guihaia,2011,31(1):43-46. |
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| Authors: | CHEN Guo-Qing |
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| Institution: | College of Life Science, Hubei Normal University, Huangshi 435002, China |
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| Abstract: | 6745 ESTs of saffron crocus in the public database of NCBI were downloaded and analysed,resulting in 1431 non-redundant ESTs with total length about 612.01 kb.A total of 108 SSRs distributed in 103 ESTs were detected,accouting for 7.55% of the non-redundant ESTs.The average distribution distance of these EST-SSRs was about 5.67 kb.Dinucleotide and trinucleotide repeats were the main types in Crocus sativus,accouting for 30.56% and 37.96% of all the SSRs,respectively.AG/CT and AAG/CTT are the most frequent motifs,accounting for 66.67% and 29.27% in dinucleotide and trinucleotide repeats,respectively.The present study provides a base for the development and further applification of EST-SSR markers in saffron crocus. |
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| Keywords: | EST EST-SSR |
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