红曲霉菌液相培养高产色素低产桔霉素的代谢调控研究

从8株固态发酵高产红曲色素菌株中筛选出一株适合于液相培养工艺的高产红曲色素菌株FL1,并对其液相培养过程特性及高产色素低产桔霉素的代谢调控策略进行了研究.发酵过程动力学研究结果表明,红曲色素与桔霉素作为次级代谢产物,其合成与红曲霉菌生长的关系属于非生长偶联型.本文首次采用连续补料的Fed-Batch培养技术,用于红曲霉...

Metabolic regulation of liquid-phase culture of Monascus for high-yielding pigment and low-yielding citrinin

FL1, the high Monascus pigment producing strain for liquid-phase culture was screened form 8 high Monascus pigment producing strains in solid-phase culture. The liquid-phase culture characteristics and metabolic regulation strategy for high-yielding pigment and low-yielding citrinin of FL1 were studied. Results of kinetics studies showed that the Monascus pigment and citrinin, as. secondary metabolites, the relationship between their synthesis and the growth of Monascus was non-growth-coupled. The fed-batch culture technology was first used as a new method in liquid-phase culture. The biomass specific growth rate of Monascus achieved 0.21 h^-1 in growth phase; in mid and late period, reducing sugar concentration could be controlled at 5 - 10 g/L by continuous feed. Thereby, the biomass specific growth rate of Monascus could be controled within a suitable range（0.02 h^-1）. The fed-batch technology enhanced pigment accumulation time and greatly increased the pigment production. This new culture technology had a good reproducibility and controllability. The average Monascus pigment yield rate and space time yield rate could be achieved 5 400 U/g starch and 5 625 U/（h·L）, obviously more than those reported at home and abroad.