| 头孢菌素C去乙酰基酶产生菌的筛选、酶学性质及产酶条件优化 |
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| 引用本文： | 韩云宾,周文瑜,胡媛,魏东芝,沈亚领,方善综,金雄熊,叶凤起. 头孢菌素C去乙酰基酶产生菌的筛选、酶学性质及产酶条件优化[J]. 工业微生物, 2011, 41(2): 1-6. DOI: 10.3969/j.issn.1001-6678.2011.02.001 |
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| 作者姓名： | 韩云宾 周文瑜 胡媛 魏东芝 沈亚领 方善综 金雄熊 叶凤起 |
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| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,鲁华生物技术研究所,上海,200237
2. 浙江永宁药业股份有限公司,浙江台州,318020 |
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| 基金项目： | 上海市重点学科建设项目资助 |
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| 摘 要： | 以7-ACA为底物从土壤中筛选得到一株产头孢菌素C乙酰水解酶的微生物,初步鉴定为红酵母.酶学性质研究表明:静息细胞CAH的最适pH为7.0,最适反应温度为25 ℃;在温度低于40℃保存静息细胞时CAH的稳定性很好,在pH 5.5～8.0的范围内保存静息细胞时CAH比较稳定.产酶条件优化结果为:葡萄糖30g/L,国产酵母...
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| 关 键 词： | 7-氨基头孢烷酸 去乙酰基-7-氨基头孢烷酸 头孢菌素C乙酰水解酶 |
Screening and identification of CAH-producing strain,enzyme properties of cephalosporin-C deacetylase and optimization of enzyme production |
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| HAN Yun-Bin,ZHOU Wen-Yu,HU Yuan,WEI Dong-Zhi,SHEN Ya-Ling,FANG Shan-Zong,JIN Xiong-Xiong,YE Feng-Qi. Screening and identification of CAH-producing strain,enzyme properties of cephalosporin-C deacetylase and optimization of enzyme production[J]. Industrial Microbiology, 2011, 41(2): 1-6. DOI: 10.3969/j.issn.1001-6678.2011.02.001 |
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| Authors: | HAN Yun-Bin ZHOU Wen-Yu HU Yuan WEI Dong-Zhi SHEN Ya-Ling FANG Shan-Zong JIN Xiong-Xiong YE Feng-Qi |
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| Affiliation: | 1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology Shanghai 200237; 2. Zhejiang Yongning Pharmaceutical Co. Ltd, Zhejiang province Taizhou 318020) |
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| Abstract: | When 7-aminocephalosporanic acid （7-ACA） was used as a single carbon source in the enrichment culture medium for screening 7-ACA-degrading microorganisms, a strain producing CAH with high yield was obtained. It was identified as red yeast. The enzyme was most active at 25℃ and pH 7.0. The enzyme was stable at temperatures lower than 40℃ and within pH 5.5-8.0. The optimized conditions for enzyme production were as followings： glucose 30 g/ L, homemade yeast 20 g/L and KH2PO45 g/L, while the initial medium pH was 7.0. |
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| Keywords: | 7-aminocephalosporanic acid deacetyl 7-aminocephalosporanic acid cephalosporin-C deacetylase |
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