Extraction of chitin and chitosan from housefly,Musca domestica,pupa shells |
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| Authors: | Min‐Woo Kim Yeon Soo Han Yong Hun Jo Myung Hyo Choi Seung Ho Kang Sun‐Am Kim Woo‐Jin Jung |
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| Institution: | 1. Department of Agriculture Chemistry, Institute of Environmentally Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju, South Korea;2. Division of Plant Biotechnology, Institute of Environmentally Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam, National University, Gwangju, South Korea;3. Korea Beneficial Insects Lab. Co. Ltd, Gokseong‐gun Jeollanam‐do, South Korea;4. Jeonnam Bioindustry Foundation Bio Control Research Center, Gokseong, South Korea |
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| Abstract: | Chitins and chitosans are some of the most abundant natural polysaccharide materials, and are used to increase innate immune response and disease resistance in humans and animals. In this work, chitin and chitosan from housefly, Musca domestica, pupa shells were obtained by treatment with HCl and NaOH. For chitin extraction, 2 N HCl and 1.25 N NaOH solutions were used to achieve decalcification and deproteinization, respectively. For chitosan extraction, 50% NaOH solution was used to achieve deacetylation. The yields of chitin and chitosan from pupa shells of M. domestica were 8.02% and 5.87%, respectively. The deacetylations of chitosan (from chitin C1 and C2) were 89.76% and 92.39%, respectively, after the first alkali treatment with 50% NaOH (w/w) solution at 105 °C for 3 h and 5 h, respectively. The viscosities of the chitosans (from chitin C1 and C2) were 33.6 and 19.2 cP, respectively. |
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| Keywords: | chitin chitosan housefly Musca domestica pupa shells |
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