Intercellular Localization of Acid Invertase in Tomato Fruit and Molecular Cloning of a cDNA for the Enzyme |
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| Authors: | Sato, Takahide Iwatsubo, Tomoyoshi Takahashi, Michiko Nakagawa, Hiroki Ogura, Nagao Mori, Hitoshi |
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| Affiliation: | 1Division of Bioproduction Science, Faculty of Horticulture, Chiba University 648 Matsudo, Chiba, 271 Japan
2Division of Biological Regulation, National Institute for Basic Biology Okazaki, 444 Japan |
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| Abstract: | The localization of acid invertase (AI, EC 3.2.1.26[EC]) in tomatofruits was studied. AI was localized in the intercellular fraction(cell wall fraction). A cDNA encoding a wall-bound form of AIfrom tomato fruits was cloned and its nucleotide sequence wasdetermined. The cloned cDNA was 2363 base pairs long and containedan open reading frame of 1908 base pairs which encoded a polypeptideof 636 amino acids. RNA blot analysis indicated that the mRNAfor the acid invertase was about 2.5 kb in length. The levelsof the mRNA were low at the mature green stage but increasedduring ripening of fruit. (Received July 13, 1992; Accepted December 3, 1992) |
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