Diversity of H2/CO2-Utilizing Acetogenic Bacteria from Fecesof Non-Methane-Producing Humans |
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Authors: | Annick Bernalier Violaine Rochet Marion Leclerc Joël Doré Philippe Pochart |
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Institution: | (1) Laboratoire de Nutrition et Sécurité Alimentaire, INRA, domaine de Vilvert, 78 352 Jouy en Josas Cedex, France , FR;(2) Conservatoire National des Arts et Métiers, 75003 Paris, France , FR |
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Abstract: | The purpose of this work was to study H2/CO2-utilizing acetogenic population in the colons of non-methane-producing individuals harboring low numbers of methanogenic
archaea. Among the 50 H2-consuming acetogenic strains isolated from four fecal samples and an in vitro semi-continuous culture enrichment, with H2/CO2 as sole energy source, 20 were chosen for further studies. All isolates were Gram-positive strict anaerobes. Different morphological
types were identified, providing evidence of generic diversity. All acetogenic strains characterized used H2/CO2 to form acetate as the sole metabolite, following the stoichiometric equation of reductive acetogenesis. These bacteria were
also able to use a variety of organic compounds for growth. The major end product of glucose fermentation was acetate, except
for strains of cocci that mainly produced lactate. Yeast extract was not necessary, but was stimulatory for growth and acetogenesis
from H2/CO2.
Received: 28 December 1995 / Accepted: 30 January 1996 |
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